I am interested in biomolecule analysis for biomedical application and biomedicine science study. From 2011 to 2016, I studied the biomolecule analysis using capillary electrophoresis (CE) technology. CE-based size sieving mechanism was investigated and then applied to clinical diagnose. (Sens. Actuators B Chem. 2018, 258, 263; J. Sep. Sci. 2016, 39(5), 986; Electrophoresis 2015, 36(14), 1651) From 2016 to 2018, with the purpose to promote CE in real life, methods to improve the robustness and precision of homemade quantitative CE (QCE) detection were studied. We developed an online fluorescence detection method to avoid the impact from analytes’ inequivalent photobleaching (Fig. 1), which was further applied to improve the accuracy of fluorescence detection based QCE. (Sens. Actuators B Chem. 2020, 319, 128035)

Since 2018, I gained interests in developing new analytical materials for biomolecule detection. Delicate materials may enhance the separation performance or even realize new functional analysis, thereby, save the analysis from hours, complex protocols, and cumbersome instrumentation. As one of our trials, we have developed a size sieving material using a copoly(PEGDA/PEGA) hydrogel. (ACS Appl. Polym. Mater. 2020, 2(9), 3886) Results showed that only 4 min will be cost for a high resolution separation using an optimized copoly(PEGDA/PEGA) hydrogel, while using conventional poly(acrylamide) hydrogel would take 28 min for the same resolution (Fig. 2). Besides, we studied the development of molecularly imprinted polymers, a highly specific molecular recognition for native proteins was achieved.